|
KMID : 0614020000160010032
|
|
Journal of Pharmaceutical Sciences (C.N.U.)
2000 Volume.16 No. 1 p.32 ~ p.38
|
|
|
Analysis of Pravastatin from Human Plasma by Ion-pair Chromatography
|
|
Lee Soon-Hee
Lim Hwan-Mi
Linh Pharn-Duan
Kang Jong-Seong
|
|
|
Abstract
|
|
|
|
A new HPLC method for the determination of pravastatin, a HMG-CoA reductase inhibitor, in human plasma is described. Sample preparation and separation on a reversed-phase column were carried out by the principle of ion-pair formation of pravastatin with alkyl amines as ion-pair reagent. Pravastatin and triamcinolone acetonide (internal standard) were extracted from human plasma with chloroform in the presence of 0.2 mM tetrabutyl ammonium ion and separated on a C_18 column with 30% acetonitrile in phosphate buffer (pH 6, 10 mM) containing 2 mM tetraoctyl ammonium ion. The limit of determination in plasma was 5 ng/ml.
|
|
|
KEYWORD
|
|
|
Pravastatin, ion-pair chromatography, alkyl amine
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|